2005 Symposium on Advanced Wound Care | |
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Laboratory Research
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A histologic evaluation of the effects of a polysulfated glycosaminoglycan* in conjunction with an occlusive or non-occlusive dressing in preparing granulation tissue for re-epithelialization Robert Blumenstein, PhD, Boyce Jubilan, DVM, PhD, Kathleen Ehrhardt, PA-C, Kyra Lezinsky, BS, Jessica Gallagher, BS, DeSales University, Center Valley, PA Following dermal injury and responding to wound environment, granulation tissue forms a necrobiotic layer that influences nutritional support to the migrating epidermis. This study investigated the effects of using a polysulfated glycosaminoglycan (PSGAG)* in conjunction with an occlusive polyurethane film (PUF)† or non-occlusive dressing‡ on density of the necrobiotic layer and rate of closure of full-thickness porcine (Yorkshire) wounds. Wounds with occlusive PUF and non-occlusive dressings were treated daily with 0.25 ml PSGAG while untreated wounds with identical dressings served as controls. Wounds were evaluated at day eight for percent closure and ANOVA was used to analyze data. Untreated wounds with non-occlusive dressings demonstrated a denser necrobiotic layer and re-epithelialized at a slower rate (9.5%) when compared to untreated PUF dressed wounds (33%). This suggests that a denser necrobiotic layer slows epidermal migration. Collagenase produced from migrating epithelium may take longer to degrade denser necrobiotic tissue thus inhibiting nutritional support to the migrating epidermis. Wounds treated with PSGAG and PUF dressings demonstrated a necrobiotic layer that was less dense, more uniform, possessed increased vascularization and re-epithelialized significantly (P=.05) faster (63%) when compared to PSGAG and non-occlusive dressings (35%) and untreated controls. The PSGAG may contribute to this acceleration by its affinity to Platelet Derived Growth Factor (PDGF (BB)). * PSGAG*Chondroprotec®, The Hymed Group Corp., Bethlehem, PA † PUF OpSite, Smith and Nephew, United Kingdom ‡ Telfa® , Kendall Company, Mansfield, MA References Agreen MS, Mirastschijski U, Karlsmark T, Saarialho-Kere UK. Topical synthetic inhibitor of matrix metalloproteinases delays epidermal regeneration of human wounds. Exp Dermatol. 2001;10(5):337–348. De Coninck A, Draye JP, Van Strubarq A, et al. Healing of full-thickness wounds in pigs:effects of occlusive and non-occlusive dressings associated with a gel vehicle. J Dermatol Sci. 1996;13(3):202–211. Daniels JT, Limb GA, Saarialho-Kere U, Murphy G, Khaw PT. Human corneal epithelial cells require MMP-1 for HGF-mediated migration on collagen I. Invest Ophthalmol Vis Sci. 2003;44(3):1048–1055. DiColandrea T. et. al. Epidermal expression of collagenase delays wound-healing in transgenic mice. J Invest Dermatol. 1998;111(6):1029–1033. Garcia-Olivas R, Hoebeke J, Castel S, et al. Differential binding of platelet-derived growth factor isoforms to glycosaminoglycans. Histochem Cell Biol. 2003;120(5):371–382. Syrokou A, Tzanakakis GN, Hjerpe A, Karamanos NK. Proteoglycans in human malignant mesothelioma. Stimulation of their synthesis induce by epidermal, insulin and platelet derived growth factors involves receptors with tyrosine kinase activity. Biochimie. 1999;81(7):733–744. |
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