2008 SAWC/WHS Attendee Registration

2005 Symposium on Advanced Wound Care

39
Laboratory Research

A 15-day wound healing evaluation of two enzymatic debriders in necrotic split-thickness wounds in MINIPIGS.

D.W. Brett, BS, BS, MS, Smith & Nephew, Largo, FL

During development of topically applied agents it is key to gain insight into the efficacy of a given formulation in vivo. But how does one gain this insight? One way is to employ a suitable animal model. To gain this insight, the effects of two papain-urea-chlorophyllin topical debriding agents in necrotic split-thickness wounds in minipigs and non-treated control wounds were studied. Agent A & B administered topically (2 ml/wound) on a daily basis for 12 days accelerated the wound healing process by increasing debridement of necrotic tissue leading to faster granulation and re-epithelialization. Agent A and agent B both induced minimal to moderate inflammation of the wound tissue wound as well as minimal hypergranulation and hyperplastic epithelium. From this study it is clear that agent A & B by and large performed in a very similar fashion. Though minor differences in the inactive components of the two agents do exist, they did not have a significant effect on the final outcome of the study. The similarity of these two agents is also supported by the results of subjective evaluations of over 90 human wounds.


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