| 13 |
Clinical Research
|
Antibacterial activity of silver-containing dressings Dolmer M, Wound Care R&D, Coloplast A/S, Humlebaek, Denmark Introduction: Silver-containing antibacterial dressings have been marketed as the answer for difficult to heal wounds as well as antibacterial barriers. Silver has been used as an antibacterial for centuries due to its good antibacterial activity and safe profile as well as the small risk of bacterial resistance towards silver Aim: The aim of this study was to test the in-vitro antibacterial activity of some silver-containing dressings currently on the market in order to evaluate the antibacterial activity. Methods: The tested dressings are some of the currently available silver dressings on the European markets. All dressings were tested in an in vitro Zone of Inhibition (ZOI) test on a bacterium detrimental to wound healing, Ps. aeruginosa. Suspensions with app. 105 CFU/ml were plated on Iso Sensitest agar plates. Ø10 mm of test products were placed on the challenged agar plates (in triplets) and the plates were incubated at 37 degrees Celcius for 24 hours. The ZOI were measured and recorded. ZOI describe the clear zones that form if the product inhibits bacterial growth. The test products were then transferred to a new agar plate with fresh challenge bacteria and incubated another 24 hours and the zones of inhibition were read again. This procedure was followed for 7 days. Results: Test results show a great difference between antibacterial activities for the tested dressings. Some dressings show only ZOI under or slightly beyond the product area, whereas others show ZOI larger than the product area. The duration of the antibacterial activity differs from dressing to dressing from 1 day (a) over 2 days (b-c-d), 3 days (e-f) to 7 days (g-h-i). Conclusions: Results show that currently available silver dressings on the European markets differ widely in their in-vitro antibacterial activity. Also the duration of antibacterial activity differs among the tested dressings in this in-vitro test a) Atraumanª Ag.b) Urgutülª S.Ag.c) Actisorbª Plus 25.d) AQAUCELª Ag.e) Polymemª Silver.f) SilverCelª.g) Contreetª Foam.h) Acticoatª 7.i) Acticoatª Absorbent |
|