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Laboratory Research
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ORC/collagen matrix containing silver controls bacterial bioburden while retaining dermal cell viability B. Cullen, Claire Boyle, T Rennison, S Gregory,.Johnson & Johnson Wound Management, Gargarve, North Yorkshire BD23 3RX, UK It is generally well accepted that all chronic wounds contain bacteria, however colonization of the wound does not necessarily indicate infection, and treatment with antimicrobials alone does not promote healing. Historically, silver containing therapies have been used to help manage bioburden and delays in healing even when clinical signs of infection are not obvious. However, while silver has a broad spectrum of activity against bacteria, the cytotoxic effect on host cells may impede wound repair. A therapy which addresses bioburden yet also retains cell viability would be useful in cases when infection is questionable. This may help reduce the incidence of wound infection without impacting rate of healing..In this study, silver dressings including an ORC/collagen matrix containing 1% silver-ORC, were assessed for their effects on the viability of cultured human dermal fibroblasts. Antimicrobial efficacy was also assessed in vitro, using a Log10 reduction assay, and confirmed by zone of inhibition testing. .ORC/collagen matrix containing 1% silver-ORC was effective against both common wound pathogens, and resistant strains such as clinical isolates of vancomycin-resistant Enterococci (VRE) and methicillin-resistant Staphylococcus aureus (MRSA). It also enhanced host cell growth, in contrast to other silver-releasing dressings tested. These in vitro results suggest that this matrix is an ideal therapy for non-healing wounds when the level of bacterial bioburden is unknown and clinical signs of infection are not obvious .. |
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