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Laboratory Research
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Ability of advanced wound therapies to modify the chronic wound environment. B. Cullen, PhD, C. Boyle, MSc, A. Essler, BSc, Johnson & Johnson Wound Management, Gargrave, North Yorkshire, UK Wound repair is a well-orchestrated progression of events, with each event employing a delicate balance of degradative and stimulatory processes. An imbalance in any of these processes can lead to an environment that is detrimental to healing. . .Previous studies have shown that the use of biomaterials that redress the balance of degradative and stimulatory factors in the chronic wound environment may be beneficial in the treatment of chronic wounds. In this in vitro study we compared the ability of collagen/ORC and other advanced wound therapies to modulate the wound environment. This included evaluation of their affect on protease activity as well as fibroblast proliferation in vitro. These therapies were also assessed by their ability to modify chronic wound exudate, which has been previously proven to be detrimental to fibroblast proliferation. .Results demonstrate that collagen/ORC significantly reduced the levels of neutrophil derived elastase and total matrix metalloproteinase activity in vitro. Data also illustrates that pretreatment of chronic wound fluid with collagen/ORC has a positive effect on fibroblast proliferation when compared to no pretreatment. This indicates that treatment with collagen/ORC is able to negate the detrimental effect of chronic wound fluid on fibroblast proliferation, an ability not observed in other advanced therapies. In conclusion, this study provides evidence that suggests that collagen/ORC, provided an optimal wound environment and therefore may be beneficial in the treatment of chronic wounds.. |
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