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Laboratory Research
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Sustained antimicrobial activity and protease inhibition in a moist wound dressing designed to speed wound healing B. Liesenfeld, QuickMed Technologies, Gainesville, FL; G. Schultz, University of Florida, Gainesville, FL, QuickMed Technologies, Gainesville, FL; W. Toreki, QuickMed Technologies, Gainesville, FL; R. Carr, QuickMed Technologies, Gainesville, FL; D. Moore We have been developing a dressing to speed healing of both chronic and acute wounds. The healing of wounds can be delayed by bacterial colonization, and by inflammation-induced protease activity in the wound. Our dressing is designed to enable optimal wound healing by providing a moist wound healing environment with antimicrobial protection as well as protease inhibiting properties through sustained delivery of an antibiotic (doxycycline) that also acts as a protease inhibitor. The dressing was prepared using a carboxymethyl cellulose (CMC) base material, structurally stabilized with a polyelectrolyte, to which doxycycline has been added in a sustained release formulation. Sustained release of doxycycline from this matrix has been demonstrated to suppress bacterial growth for 14+ days in experiments featuring daily reinoculation of 15 mm squares of matrix with 500 µl of 106 cfu/ml of either Staphylococcus aureus or Escherichia coli. Azocoll assays on clostridial collagenase (a protease) solution, demonstrated a 6-8 fold reduction in activity when treated with the QMT dressing compared to untreated CMC dressings. Additional experiments are underway on chronic wound fluids. The polyelectrolyte stabilized dressing itself has been demonstrated to have antimicrobial properties independent of doxycycline, and has proven efficacious against a wide range of species including MRSA and VRE. |
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