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Laboratory Research
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Glycosaminoglycan composition of granulation tissue from wounds treated with negative pressure wound therapy or moist wound therapy A.K. McNulty, T. Feeley, M. Schmidt, K. Norbury, K. Kieswetter Introduction: The method by which Vacuum Assisted Closure¨ Therapy (V.A.C. ¨ Therapy) affects the wound matrix remains to be elucidated. The purpose of this study was to characterize changes in hyaluronic acid and sulfonated glycosaminoglycan (sGAG) composition of granulation tissue collected from excisional porcine wounds which had been treated with either Negative Pressure Wound Therapy (NPWT)* or Moist Wound Therapy (MWT). Methods: Four, full thickness, surgical wounds (5 cm diameter) were made dorsally on twenty healthy, 3Ð6 month Yorkshire swine. Wounds were treated with either NPWT continuously at -125 mmHg, or MWT (Duoderm¨ CGF, Convatec, Skillman, NJ). After 9 days, granulation tissue was removed and enzymatically (for sGAG) or non-enzymatically (for hyaluronic acid) extracted. Hyaluronic Acid concentrations were measured using competitive ELISAs. A colorimetric assay was used for measuring sGAG concentration. Repeated measures 1-way ANOVA were used to test for differences; P<0.05 was considered statistically significant. Results: In granulation tissue, there were no significant differences between NPWT and MWT for sGAGs. However, hyaluronic acid concentrations were significantly higher in NPWT granulation tissue (P<0.05). Discussion: Hyaluronic acid is produced in large amounts during wound healing where it is believed to support cell migration and proliferation. The increased levels of hyaluronic acid observed with NPWT versus standard moist wound healing may therefore be beneficial to wound healing. While total sGAG concentrations were analyzed in this study, it may be more important to look at individual sGAG such as dermatan sulfate. Further work may look at levels of hyaluronic acid over time and follow the wound to epithelialization *V.A.C.¨ GranuFoam¨ Dressing and V.A.C.¨ SystemTM (Kinetic Concepts, Inc., San Antonio, TX) Longaker, et al. Ann Surg. 1991;213(4): 292Ð296 Mast, et al. Surg Gynecol Obstet. 1992;174:441Ð451 Trowbridge, et al. J Biol Chem. 2002;277(45):42815Ð42820 |
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