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Laboratory Research
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Absorption of PDGF into small intestinal submucosa (SIS*): an innovative treatment for difficult-to-heal and chronic wounds Edith S. Nihsen, MS, David A. Zopf, MS, David M.J. Ernst, BS, Chad Johnson, PhD, Michael C. Hiles, PhD Difficult to heal and chronic wounds present a significant clinical challenge. Bioactive materials can be useful in the treatment of such wounds, shifting the static wound environment to a more active acute phase. One such biomaterial is a naturally derived scaffold consisting of porcine small intestinal submucosa (SIS*). Clinical studies have shown that treatment with SIS* significantly increased healing of chronic ulcerative wounds. SIS* contains bioactive forms of the growth factors transforming growth factor 1 (TGF-beta1) and basic fibroblast growth factor (FGF-2), before and after sterilization with ethylene oxide. SIS* may also absorb and protect the activity of growth factors, such as PDGF, not endogenous to SIS*, but present in the wound bed. The present study examined the ability of SIS* to bind, retain, and protect recombinant human PDGF in vitro. Recent experiments demonstrated the capacity to absorb and retain a significant amount of PDGF-BB after incubation (0 ng/g initial versus 5.64 ± 2.4 ng/g post-incubation, P<0.05) in an aqueous PDGF-BB solution and elution (3.10 ± 0.7 ng/g versus initial SIS*, P<0.005) with high purity water, as determined by ELISA. Additional experiments demonstrated this capacity in the protein-rich environment of a PDGF-BB-spiked serum, while protection experiments determined that binding to SIS* decreased PDGF degradation by plasmin. The present study supports the hypothesis that SIS* can bind, stabilize, and protect bioactive PDGF-BB and suggests that this binding may occur in vivo with endogenous growth factors in the wound bed *Oasis¨ Wound Matrix, manufactured by Cook Biotech Incorporated, West Lafayette IN, distributed exclusively by Healthpoint Ltd., Fort Worth, TX. Demling RH, Niezgoda JA, Haraway GD, Mostow EN. WOUNDS. 2004;16(1):18Ð22 Brown-Etris M, Cutshall WD, Hiles MC. WOUNDS. 2002;14(4):150Ð166 Voytik-Harbin SL, Brightman AO, Kraine MR, Waisner B, Badylak SF. J Cell Biol. 1997;67: 478Ð491 McDevitt CA, Wildey GM, Cutrone RM. J Biomed Mater Res. 2003;(67A):637Ð640. |
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