| 252 |
Clinical Res.
|
Sensitivity of silver resistant bacteria to silver containing wound dressings Steven Percival, PhD, Moses K. Nutekpor, Jayne Dolman, Philip G. Bowler, Christine A. Cochrane, ConvaTec Wound Therapeutics, Deeside, Flintshire, UK The aim of this study was to determine if silver containing dressings were effective in killing silver-resistant bacteria (sil positive) known to have been associated with chronic wounds. The antimicrobial dressings used in this study were nanocrystalline* and hydrofiber dressings$. Clinical isolates grown on fresh Mueller Hinton agar (MHA) were separately prepared in Maximal Recovery Diluent (MRD) at a concentration of approximately 1 x 105 colony forming units/ml. A 1 ml volume of each organism suspension was inoculated and evenly spread across the surface of the agar plate. 2 cm diameter circular sections of dressings were aseptically cut and with the use of sterile forceps, one circle of each dressing type was placed onto the MH agar plates and pressed down to maximise contact. A negative control plate for each challenge organism was included. The hydrofiber dressings were hydrated with MRD and the nanocrystalline dressing was hydrated with sterile distilled water. All plates were then incubated at 35oC for 24 h. Following incubation, all plates were observed to determine the antimicrobial activity of the dressing and the corrected zones of inhibition were measured. The results from this study showed that the two silver-containing dressings tested exhibited similar levels of activity against silver resistant (sil positive) wound isolates, with efficacy demonstrated against two out of three strains of Enterobacter cloacae. *Acticoat (Smith and Nephew).$AQUACEL¨ Ag (ConvaTec Wound Therapeutics) |
|