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Lab Research
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Autoclave versus cetylcide II versus glass bead sterilizer: which is most effective? First Author: Michael Corcoran Authors: Michael Corcoran, Stephanie Wu, Jan Vanik The purpose of this study is to determine the efficacy of Glass Bead sterilization in comparison to the Autoclave, the gold standard of sterilization, and Cetylcide II, the use of which is widespread in clinical practices. Bacterial colonies were introduced to sterile tripticase soy broth and were allowed to incubate over night. Several instruments were soaked in tripticase soy broths contaminated with various organisms, including S. aureus, S. epidermidis, P. aeruginosa, and E. coli. Contaminated instruments were sterilized by one of the three previously mentioned methods and then plated on Sheep Blood Agar to indicate the presence of residual bacteria.No bacterial growth was present on plates of instruments sterilized by Autoclave or the Glass Bead Sterilizer. Growth of S. aureus and S. epidermidis was present on plates of instruments sterilized by Cetylcide II. Glass Bead sterilization provides an effective method of instrument sterilization for clinical use. Glass Bead sterilization is more effective than Cetylcide II and as effective as autoclaving, but only takes a fraction of the time required by autoclaving or soaking in Cetylcide II. |
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