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Oral Abstracts (Session 1 of 5)
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Moderator: Patricia M. Mertz, BA (Presentation 32.1) A noninvasive electric magnetic field device accelerates skin keratinocyte migration Jie Li, MD, PhD; Charles Q. Ma; James J. Wu; Ya-Xiong Tang, PhD, University of Miami, Miami, Fla; Machael Spiegel, PhD, Advatech Corporation, Miami, Fla Abstract: In this study, we evaluated the effect of a noninvasive electric magnetic field device, Field Therapy Accelerator (FTA), on skin re-epithelialization by measuring keratinocyte migration in an in-vitro incisional wound model. Normal human epidermal keratinocytes were grown into confluent. At time 0, a cross-shaped wound gap was created among monolayer cells in the center of the culture dish. Cells were then treated with FTA for 1 hour immediately after wounding and 1 hour every 24 hours for a total of 4 hours in 4 days. The cell cultures were examined at time 0 and every 24 hours thereafter for a total of 10 days. A phase-contract microscope with a digital camera system was used to capture the images. Cell migration was evaluated and quantified by the time and percentage of the wound gap covered by the cells that migrated in and filled the gap. The FTA treatment significantly accelerated keratinocyte migration. The wound gaps completely filled 6 days after wounding, 3 days earlier than control group (p<0.001). Cell migration is a key step leading to skin re-epithelialization. This study suggests that electric magnetic field stimulation may improve wound re-epithelialization process via promoting cell migration. |
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