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Oral Abstracts (Session 1 of 5)
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Moderator: Patricia M. Mertz, BA (Presentation 32.5) HSP70 is proinflammatory in vivo: implications for chronic wound healing Caesar Anderson, MD; George Perdrizet, MD, PhD, FACS; Michael Rewinski, Center for Wound Healing and Hyperbaric Medicine, Hartford Hospital, Hartford, Conn; Roli Kumar, MS; Martin Berman, MD; Lawrence Hightower, PhD, Molecular and Cell Biology, Department of Pathology, Hartford Hospital, UCHC and the University of Connecticut, Storrs, Conn Introduction: Failure to down regulate inflammatory responses is often used to explain why chronic wounds fail to heal. Might the persistent expression of stress proteins lead to chronic inflammation in the wound bed? We tested the hypothesis that stress proteins will cause acute inflammatory swelling in an in-vivo murine model. Methods: C3H mice (n=57) were divided into 6 groups based on the protein they received by subcutaneous injection: Group 1 serum, Group 2 heat-treated serum (60&Mac251;C x 45min), Group 3 HSP70 plus serum, Group 4 HSP70 plus heat-treated serum, Group 5 GRP78 plus serum, and Group 6 GRP78 plus heated-serum. Ear thickness was measured (mm) at 24 and 48 hours after injections. Specific ear swelling was determined and the means of the differences reported. Comparisons between groups were preformed using Dunnet’s T3 procedure, significance p<0.05. Results: Stress proteins are associated with significantly increased mean swelling (hsp70 = 8mm) in comparison to injections of saline (S = 3mm) or autologous serum (AS = 4mm). Conclusions: Stress proteins are pro-inflammatory in vivo and may set the stage for persistent inflammation within the chronic wound bed. |
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