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Oral Abstracts (Session 3 of 5)
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(Presentation 34.3) Noninvasive electric magnetic fields: effects on keratinocyte migration and proliferation Juan Chen, MD; Ran Huo; Qianli Ma; James J. Wu; Maria E. Miyar, BS; Stephen C. Davis; Jie Li, MD, Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, Fla Though evidence has shown that very small electric currents produce a beneficial therapeutic result for wounds, clinical application has been hindered by lack of understanding of its mechanism of action and its invasive nature. In this study, the authors evaluated the effects of a noninvasive electric magnetic field (EMF) device on wound epithelization in both in-vitro and in-vivo models. Using an in-vivo porcine second-degree burn model, the authors found that exogenous electric magnetic fields can stimulate epithelization. Further in-vitro analyses with human skin keratinocyte cultures demonstrated that this noninvasive EMF device has a very strong effect on accelerating keratinocyte migration and a relative weaker effect on promoting keratinocyte proliferation. Subsequent studies with dermal fibroblasts showed that the effects of the EMF on cell migration and proliferation seem keratinocyte specific without such effects on dermal fibroblasts. The authors’ most recent gene expression analysis of GeneChip probe microarray of keratinocytes revealed that the EMF treatment significantly increases the expression of genes involved in signal transduction and regulation of cell attachment and migration, such as homeo box C8, A-kinas anchor protein 7, caldesmon 1, CDC2-related protein kinase 7, and Eph receptor B2. This study suggests that noninvasive electric magnetic field accelerates wound reepithelization through a mechanism of promoting keratinocyte migration and proliferation. References Bassett CAL, Pawluck RJ, Becker R. Effects of electric currents on bone formation in vivo. Nature. 1964;204:652. Davis SC, Ovington LG. Electrical stimulation and ultrasound in wound healing. Dermatol Clin. 1993;11:775–781. Hinsenkamp M, Jercinovic A, Graef C, Wilaert F, Heenen M. Effects of low frequency pulsed electrical current on keratinocytes in vitro. Bioelectromagnetics. 1997;18:250–254. Illingworth CM, Barker AT. Measurement of electrical currents emerging during the regeneration of amputated fingertip in children. Clin Phys Physiol Meas. 1980;1:87–89. Li J, Kirsner RS. Wound Healing. In: Surgery of the Skin. Elsevier Mosby; 2005:97–115. |
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